Figure 2.

Reduction of the enhancer activity of an RLS-risk common polymorphism of the regulatory locus of the gene MEIS1 in the mouse ganglionic eminence. A. Transgenic mouse embryos after injection of the protective (left, ‘A’ for alanine) and risk allele constructs (right, ‘G’ for guanine). The blue color indicates the regions expressing the reporter gene beta-galactosidase, and the arrowheads indicate the telencephalic signal in the ganglionic eminence. B. Analysis of the signal intensity and volume demonstrated a significant reduction (*) of the enhancer activity of the risk versus the protective construct, down to 35% and 24%, respectively. Reproduced and modified with permission from Spieler and others 2014.