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. 1999 May;181(9):2846–2851. doi: 10.1128/jb.181.9.2846-2851.1999

FIG. 1.

FIG. 1

Overexpression and purification of recombinant SigB and RsbW proteins. SDS–10% PAGE gel analysis (25) of fractions from the purification of SigB and RsbW. Lane M contains the molecular mass markers (masses in kilodaltons are shown on the left). Cell extracts from E. coli BL21(DE3) harboring pEM102 (Trx-SigB overexpression) grown without IPTG induction (lane a), in the presence of IPTG (lane b), and the affinity-purified Trx-SigB fusion protein (lane c) are shown. Cell extracts from E. coli BL21(DE3) harboring pEM202 (Trx-RsbW overexpression) grown without IPTG induction (lane d), in the presence of IPTG (lane e), and with the affinity-purified Trx-RsbW fusion protein (lane f) are shown.