FIG. 6.

SigB-directed transcription of asp23 and coa and inhibition by RsbW. The transcription reaction mixtures contained 0.4 U of E. coli core RNA polymerase and 0.02 μg of DNA templates containing the putative promoter region of asp23 (lanes a to d) and coa (lanes e to h). Lanes: a and e, no addition; b and f, 0.58 μg of Trx-tagged SigB that had been preincubated with dialysis buffer; c and g, 0.58 μg of Trx-tagged SigB that had been preincubated with 0.36 μg of Trx-RsbW fusion protein; d and h, 0.58 μg of Trx-tagged SigB that had been preincubated with an excess amount (18 μg) of bovine serum albumin. The products of transcription were subjected to electrophoresis on a 6% denaturing polyacrylamide gel and visualized by autoradiography. The arrows indicate the positions of the anticipated transcripts. Lane M, ³²P-labeled DNA size markers as in Fig. 3.