FIG. 5.

Secretion of YopM by yscP Y. pestis. An immunoblot analysis of YopM secreted from Y. pestis KIM5-3001 (parent), KIM5-3001.17, (ΔP1), KIM5-3001.18 (ΔP2), and the mutants carrying yscP in trans on pYscP.2 or pYscP (/P and /P^T7, respectively) is shown. Bacteria were grown in TMH at 37°C without Ca²⁺. Proteins from the culture medium were separated by SDS-PAGE (12% polyacrylamide gels), transferred to Immobilon P, and visualized with antibody specific to YopM and a secondary antibody conjugated to alkaline phosphatase.