FIG. 7.

Evidence of an independent promoter for yscP. An immunoblot analysis of the Pla⁻ Lcr⁺ parent strain of Y. pestis (KIM8-3002, parent) and the Pla⁺ ΔP1 Y. pestis KIM5-3001.17 carrying yscP in trans on pYscP.2 or pYscP (ΔP1/P and ΔP1/P^T7, respectively) is shown. Bacteria were grown in TMH without Ca²⁺. Proteins from the whole-cell (wc) and culture medium (e) fractions were separated by SDS-PAGE and transferred to Immobilon P. They were detected by using polyclonal antibody specific to the fusion protein GST-YscP and a secondary antibody conjugated to horseradish peroxidase and were visualized by enhanced chemiluminescence.