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. Author manuscript; available in PMC: 2022 Aug 12.
Published in final edited form as: Methods Enzymol. 2021 Jul 29;658:379–406. doi: 10.1016/bs.mie.2021.06.011
5 × Charging buffer, pH 7.6 10 × Translation buffer
Reagent Final concentration Reagent Final concentration
HEPES 0.5 M Tris-Cl pH 7.5 0.5 M
KCl 0.05 M NH4Cl 0.7 M
MgCl2 0.1 M KCl 0.3 M
H2O to 1 mL MgCl2 0.07 M
BME 0.05 M
H2O To 1 mL