. Author manuscript; available in PMC: 2022 Aug 12.

Published in final edited form as: Methods Enzymol. 2021 Jul 29;658:379–406. doi: 10.1016/bs.mie.2021.06.011

Table 3.

EF-Tu/Ts mixture for miscoding kinetic assays

Reagent	Concentration in mixture	Final reaction concentration
Water	‒	to 500 μL
10 × Translation Buffer	1.1 ×	1×
GTP	1.11 mM	1 mM
MgCl₂	7.77 mM	7 mM
EF-Tu	44.44 μM	40 μM
EF-Ts	11.11 μM	10 μM
PEP	3.33 mM	3 mM
PK	0.11 mM	0.1 mM