Buffer R-A		Buffer R-D		Buffer R-O (10 x)
Reagent	Final concentration	Reagent	Final concentration	Reagent	Final concentration
Tris HCl, pH 7.5	20 mM	Tris HCl, pH 7.5	20 mM	Tris HCl, pH 7.5	200 mM
NH4Cl	100 mM	NH4Cl	500 mM	NH4Cl	600 mM
MgCl2	10 mM	MgCl2	10mM	MgCl2	52.5 mM
EDTA	0.5 mM	EDTA	0.5 mM	EDTA	2.5 mM
BME	6mM	Sucrose	1.1 M
Buffer R-10: 1 × Buffer R-O		Buffer R-40		Buffer R-50
Reagent	Final concentration	Reagent	Final concentration	Reagent	Final concentration
Buffer R-O (10 x)	1x	Buffer R-O (10 x)	1x	Buffer R-O (10 x)	1x
Sucrose	10% (w/v)	Sucrose	40% (w/v)	Sucrose	50% (w/v)
BME	3 mM	BME	3 mM	BME	3 mM
Buffer R-60
Reagent					Final concentration
Buffer R-O (10 x)					1 x
Sucrose					60% (w/v)
BME					3mM