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. 2022 Jul 29;13:932722. doi: 10.3389/fimmu.2022.932722

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Copyright © 2022 Dong, Mu, Kong, Qin, Zhou, Wang, Cheng, Luo, Ai and Xu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Safety evaluation of oral administration of CyHV-2 yeast vaccine. (A, C, E) Histological examination by H&E staining of foregut (A), midgut (C), and hindgut (E) from Control fish and Vaccine fish and Bo-vaccine fish (n = 6 fish per group). Scale bars, 50 μm. Con, control; Va, vaccine; Bo-va, booster vaccine. (B, D, F) The length-width ratio of foregut (B), midgut (D), and hindgut (F) intestinal folds in control fish, vaccine fish, and booster vaccine fish (n = 6 fish per group). Control vs. Vaccine and Bo-vaccine, ns, not significant, unpaired Student’s t-test (at 10dpv and 17dpv), one-way ANOVA with Bonferroni correction (at 27dpv and 34dpv). Data are representative of three different independent experiments (mean ± SEM).