Table 1.
Antibodies used for flow-cytometric phenotyping of bovine monocyte subsets.
| Panel 1 | Antigen | Clone/Source of mAb | Detection/Source |
|---|---|---|---|
| Core | CD14 | CAM66A/Kingfisher | Anti-IgM-PE/Southern Biotech |
| CD16 | KD1/Bio Rad | Anti-IgG2a-AF647/Molecular Probes | |
| CD172a | CC149/Bio Rad | Anti-IgG2b-AF488/Molecular Probes | |
| Phenotypic marker (Pm) | CD5 | AFRCIAH-CC29/in house | Anti-IgG1- BV421/BioLegend |
| CD8α | CACT80C/Kingfisher | ||
| CD11a | HUH73A/Kingfisher | ||
| CD40 | IL-A156/Kingfisher | ||
| CD43 | CO.44B8/Bio-Rad | ||
| CD45 | 1.11.32/Bio-Rad | ||
| CD62L | Du-1-29/in house | ||
| CD80 | IL-A159/Kingfisher | ||
| CD86 | IL-A190A/Kingfisher | ||
| CD163 | LND68A/Kingfisher | ||
| CD205 | IL-A114/Bio Rad | ||
| MHC-II | VPM54/in house | ||
| Panel 2 | Antigen | Clone/Source of mAb | Detection/Source |
| Core | CD14 | CAM36A/Kingfisher | Anti-IgG1-BV421/BioLegend |
| CD16 | KD1/Bio Rad | Anti-IgG2a-AF647/Molecular Probes | |
| CD172a | CC149/Bio Rad | Anti-IgG2b-AF488/Molecular Probes | |
| Pm | CD11c | BAQ153A/Kingfisher | Anti-IgM-PE/Southern Biotech |
| Panel 3 | Antigen | Clone/Source of mAb | Detection/Source |
| Core | CD14 | CAM66A/Kingfisher | Anti-IgM-PE/Southern Biotech |
| CD16 | KD1/Bio Rad | Anti-IgG2a-AF647/Molecular Probes | |
| CD172a | DH59B/Kingfisher | Anti-IgG1-BV421/BioLegend | |
| Pm | CD11b | MM10A/Kingfisher | Anti-IgG2b-AF488/Molecular Probes |