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. 2022 Jul 29;12:944248. doi: 10.3389/fonc.2022.944248

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Copyright © 2022 Liu, Hong, Qiu, Li, Liu, Zhu, Lai, Chen, Yang, Xu and Fang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transfection efficiency and function of CAR-T cells in the three groups. (A) CAR expression of T cells in the three groups measured by flow cytometry. (B) Transfection efficiency among the three groups. (C) Killing efficacy of Nalm6-GL by CB CAR-T, PB CAR-T, PB (patient) CAR-T, and control wild-type T cells after coculture for 18 h Results are representative of at least three independent experiments with T cells from different healthy donors. (D) Killing efficacy of K562-GL by CB CAR-T, PB CAR-T, PB (patient) CAR-T, and control wild-type T cells after coculture for 18 h. Results are representative of at least three independent experiments with T cells from different healthy donors. (E) Levels of IL-2, IL-6, IL-10, TNF-α, and IFN-γ in the supernatant after the CAR-T cells were cocultured with Nalm6-GL. *p < 0.05, **p < 0.01, ***p < 0.001.