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. 2022 Jul 29;12:944248. doi: 10.3389/fonc.2022.944248

Figure 3.

Figure 3

Changes in T-cell subsets, activation, PD-1, and LAG3 expression of CAR-T cells in the three groups after coculture with Nalm6-GL. (A) Flow cytometry analysis of TN, TCM, TEM, and TEMRA in CAR-T cells of the three groups. Right upper quadrant: naive T(CD45RA+CCR7+), left upper quadrant: TCM(CD45RA-CCR7+), left lower quadrant: TEM(CD45RA-CCR7-), and right lower quadrant: TEMRA(CD45RA+CCR7-). (B, C) Proportion of TN, TCM, TEM, and TEMRA in CAR-T cells. (B) CD4+T cells, (C) CD8+T cells, red represents TEMRA, green represents TEM, blue represents TCM, and purple represents naive T cells. (D) Flow cytometry analysis of CD69 in the three groups of CAR-T cells after coculture with Nalm6-GL for 18 h (E) Expression of CD69 in the three groups of CAR-T cells after coculture with Nalm6-GL for 18 h (F) Flow cytometry analysis of the expression of PD-1 and LAG3 in the three groups of CAR-T cells after coculture with Nalm6-GL for 72 h (G) The expression of PD-1 and LAG3 in the three groups of CAR-T cells after coculture with Nalm6-GL for 72 h. PD-1, programmed death 1; LAG3, Lymphocyte-activation gene 3; TN, Naive T cell; TCM, Central memory T cell; TEM, effector memory T cell; TEMRA, Terminal effector T cell; Treg, regulatory T cell. *p < 0.05, **p < 0.01, ***p < 0.001.