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. 2022 Jul 29;12:944248. doi: 10.3389/fonc.2022.944248

Figure 5.

Figure 5

Immunophenotype of the three groups of CAR-T cells in vivo. (A) Flow cytometry analysis of TN, TCM, TEM, and TEMRA in the peripheral blood of mice in the three CAR-T groups on day 14. Right upper quadrant: naive T(CD45RA+CCR7+), left upper quadrant: TCM(CD45RA-CCR7+), left lower quadrant: TEM(CD45RA-CCR7-), and right lower quadrant: TEMRA(CD45RA+CCR7-). (B) Proportion of TN, TCM, TEM, and TEMRA in the peripheral blood of mice in the three CAR-T groups on day 14. Red represents TEMRA, green represents TEM, blue represents TCM, and purple represents naive T cells. (C) Flow cytometry analysis of TN, TCM, TEM, and TEMRA in the spleen of mice in the CB and PB CAR-T groups on day 51. (D) Proportion of TN, TCM, TEM, and TEMRA in the spleen of mice in the CB CAR-T and PB CAR-T groups on day 51. TN, Naive T cell; TCM, Central memory T cell; TEM, effector memory T cell; TEMRA, Terminal effector T cell; Treg, regulatory T cell. *p < 0.05, **p < 0.01, ***p < 0.001.