TABLE 2.
Mature miRNA sequences analyzed in this study and related forward primers employed for qPCR. All primers were obtained from Sigma and employed at 500-μM concentration.
| Mature miRNA (Sequence 5′-3′) | Forward primer (5′-3′) | |
| miR-1 | hsa-miR-1-3p (UGGAAUGUAAAGAAGUAUGUAU) |
TGGAATGTAAAGAAGTATGTAT |
| miR-21 | hsa-miR-21-5p (UAGCUUAUCAGACUGAUGUUGA) |
TAGCTTATCAGACTGATGTTGA |
| miR-133a | hsa-miR-133a-3p (UUUGGUCCCCUUCAACCAGCUG) |
TTTGGTCCCCTTCAACCAGCTG |
| miR-208a | hsa-miR-208a-3p (AUAAGACGAGCAAAAAGCUUGU) |
ATAAGACGAGCAAAAAGCTTGT |
| Cel-miR-39 (Housekeeping spike-in gene) |
cel-miR-39-3p (UCACCGGGUGUAAAUCAGCUUG) |
TCACCGGGTGTAAATCAGCTTG |