Figure 3.

Lab4 produces a significant increase in plaque content of smooth muscle cells (αSMA‐positive) in LDLr^−/− mice fed HFD. IHC of sections from the aortic root of mice fed HFD for 12 weeks (Control) or HFD supplemented with Lab4P (Lab4P) were stained using the αSMA antibody, mounted with DAPI, or appropriate isotype control antibodies and images taken by fluorescent microscopy. Representative images are shown for control and Lab4P groups for αSMA staining (αSMA), αSMA and DAPI staining (Merge) and IgG isotype control (Isotype) (A). The plaque content of αSMA‐positive smooth muscle cells was determined using the Image J software. Data are mean ± SEM (n = 5 for Control and 4 for Lab4P). Statistical analysis was carried out using an unpaired Student's t test (*p ≤ 0.05).