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. 2021 Jul 19;65(17):2100214. doi: 10.1002/mnfr.202100214

Figure 6.

Figure 6

MCP‐1 driven migration of human monocytes was attenuated by different concentrations of CM from Lab4 or CUL66. Migration assays were carried out on THP‐1 monocytes as described in Section 4 using Lab4 CM at concentrations of 8, 6, and 4 µg mL‐1 (A) or CUL66 CM at concentrations of 5, 4, and 3 µg mL‐1 (B). Cells treated with vehicle but in the absence of MCP‐1 were also included for comparative purposes. Migration was determined as a percentage of the total input cells and displayed as fold‐change in migration relative to the Vehicle control which was arbitrarily set to 1. Data are presented as mean ± SEM from five (A) or three (C) independent experiments. Statistical analysis was performed using a one‐way ANOVA with Dunnett (2‐sided) post‐hoc test where **p < 0.01 and ***p < 0.001.