Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effects of magnesium and ammonium ions on the ability of PIMT to methylate isoaspartyl δ-sleep-inducing peptidea
| Mg2+ concn (mM) | NH4+ concn (mM) | Methylation (% control) |
|---|---|---|
| 0 | 0 | 100 |
| 4.5 | 0 | 102 |
| 22.5 | 0 | 98 |
| 0 | 25 | 96 |
| 0 | 200 | 82 |
| 22.5 | 200 | 66 |
See Materials and Methods for a description of the methyl-accepting capacity assay. Final concentrations of isoaspartyl δ-sleep-inducing peptide and PIMT were 1 and 2 μM, respectively. Reactions were done in duplicate, and the average result is reported.
