TABLE 3.
β-Galactosidase levels from lacZ fusions in wild-type and mutant strains grown in LB medium
lacZ fusiona | Relevant genotype | β-Galactosidase sp act (U/mg of protein) in cells grown in LB mediumb:
|
Glucose repression ratioc | |
---|---|---|---|---|
Without glucose | With glucose | |||
TMS922 | Wild type | 44.0 | 36.6 | 1.2 |
ptsH1 | 33.6 | 33.5 | 1.0 | |
HUT924 | Wild type | 21.3 | 4.5 | 4.7 |
ptsH1 | 20.6 | 20.0 | 1.0 | |
BGL2 | Wild type | 14.2 | 2.2 | 6.5 |
ptsH1 | 15.2 | 10.5 | 1.4 |
All strains were derivatives of strain 168 and had the indicated lacZ fusion integrated as a single copy at the amyE locus.
Data are the averages of three or more determinations, which did not vary by more than 25%. Cultures were grown in LB medium with or without glucose.
The glucose repression ratio was calculated by dividing the enzyme activity found in the culture grown without glucose by the enzyme activity found in the culture grown with glucose.