TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| N. gonorrhoeae | ||
| FA1090 | Wild type | 26 |
| FA6959 | fetA::Ω | This study |
| FA6990 | tonB::cat | This study |
| FA6991 | fetA::Ω tonB::cat | This study |
| FA7015 | fetB::Ω | This study |
| AR89 | Wild-type clinical isolate | Clinical isolate, collection of P. Klebba |
| E. coli | ||
| DH5αMCR | F−mcrA mcrB mrr j80 dlacZΔM15 Δ(argF-lac)U169 recA1 endA1 hsdR hsdM supE44 l−thi-1 gyrA96 relA1 F−thi entA pro trp rpsL | Bethesda Research Laboratories |
| BN1071 | 22 | |
| Plasmids | ||
| pUNCH170 | Partial gonococcal tonB clone | 5 |
| pUNCH320 | 5.3-kb clone containing fetA fetB | 3 |
| pUNCH666 | Contains fetA::Ω | This study |
| pUNCH1154 | Promoterless fetA clone | This study |
| pUNCH1162 | Partial fetB clone | This study |
| pUNCH1165 | Contains fetB::Ω | This study |
| pUNCH1166 | Contains fetB::Ω with gonococcal DNA uptake sequence | This study |
| pUNCH1171 | Contains partial tonB::cat | This study |
| pUNCH1173 | fetA in pUC18 | This study |
| pHP45Ω | Source of the Ω fragment | 30 |
| pCR II | TA cloning vector | Invitrogen |
| pNC40 | Source of the cat cassette | 33 |
| pUP1 | Source of the gonococcal uptake sequence | 15 |