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Molecular organization of P. denitrificans PHA biosynthesis genes and restriction endonuclease sites. (a) Restriction map of the chromosomal region surrounding the phaC locus; (b) subfragments already cloned, used as probes, obtained in this study, and registered in DDBJ; (c) restriction map of PH65 region in detail; (d) organization of ORF1, phaCPd, ORF3 (phaPPd), and ORF4 (phaRPd); and (e) structures of plasmids used for expression of phaCPd, phaPPd, and phaRPd in E. coli.
