TABLE 1.
Plasmids used in this study
| Plasmid | Relevant description | Source or reference |
|---|---|---|
| pBluescript II SK(+) | 3.0 kb; Apr; lacPOZ′; cloning vector | Stratagene |
| pTV119N | 3.2 kb; Apr; lacPOZ′; cloning vector | Takara |
| pPDES3.6 | 6.6 kb; pBluescript II SK(+) carrying 3.6-kb fragment containing phaCPd | 31 |
| pPDH6.5 | 9.5 kb; pBluescript II SK(+) carrying 6.5-kb fragment containing phaABPd | 32 |
| pPDPP7.2 | 10.2 kb; pBluescript II SK(+) carrying 7.2-kb fragment containing pha′CPRPd | This study |
| pPDPP2.6 | 5.6 kb; pBluescript II SK(+) carrying 2.6-kb fragment containing ORF1 | This study |
| pPDPA4.5 | 7.4 kb; pBluescript II SK(+) carrying 4.5-kb fragment containing pha′CPRPd | This study |
| pPDPX2.2 | 5.1 kb; pBluescript II SK(+) carrying 2.1-kb fragment containing pha′CPRPd | This study |
| pPDPK1.7 | 4.6 kb; pBluescript II SK(+) carrying 1.7-kb fragment containing pha′CPR′Pd | This study |
| pTVC | 5.2 kb; pTV119N carrying 2.0-kb fragment containing phaCPd | This study |
| pTVCP | 5.7 kb; pTV119N carrying 2.6-kb fragment containing phaCPPd | This study |
| pTVCP4 | 6.4 kb; pTV119N carrying 3.3-kb fragment containing phaCPRPd | This study |
| pBBRKm | 5.8 kb; Kmr Tra+ Mob+; broad-host-range cloning vector from pBBR1MCS (10); not belonging to InqP, InqQ, or InqW groups | 31 |
| pBBRKmAB | 7.3 kb; pBBRKm derivative containing phaABPd | This study |