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. 2022 Jul 29;13:938075. doi: 10.3389/fimmu.2022.938075

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Copyright © 2022 Sebastian, Hum, McCool, Wilson, Murugesh, Martin, Rios-Arce, Amiri, Christiansen and Loots

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of Trem2-expressing macrophages using flow cytometry. (A) Flow cytometry gating strategy to identify Trem2-expressing M2-like macrophages. (B) Flow cytometry plots showing Trem2+ M2-like macrophages at D0 and D7. (C) Proportion of Trem2+ macrophages among M2-like macrophages at D0 and D7. Data is represented as mean ± SD. (D) Proportion of Trem2 and CD206- expressing macrophages relative to all macrophages. Data is represented as mean ± SD. *p ≤ 0.05; ***p ≤ 0.001; ****p ≤ 0.0001 (two-sided unpaired t-test). (E) Genes differentially expressed between injured joints of wildtype (WT) and Trem2^-/- (KO) mice at D7.