Figure 5.

Outside of its native context, the ribosome binding site (RBS) containing AUG1 promotes translation, while the RBS containing AUG2 does not. A) Schematic representation of the translational fusion constructs used for analysis of the two potential RBSs. Chromosomal regions a, b and c, pairwise fused in plasmids pAUG1-egfp and pAUG2-egfp, are indicated. The RNA sequences of the regions b and c are shown and selected nucleotides are numbered (+1 corresponds to the metA TSS, see the Chr. scheme). Potential RBS elements (SD and start codons) are underlined. For other details, see Figure 4A and Figure 1A. B) Relative fluorescence of TY and MM cultures of S. meliloti 2011 strains carrying the indicated plasmids. The graph shows means of three independent experiments and single measure points representing means from three technical replicates of each independent experiment.