Strains |
|
MC4100 |
araD139 Δ(argF-lac)U169 thiA rpsL150 relA1 deoC1 ptsF25 rbsR flbB5301
|
33 |
MC4100F′ |
MC4100/F′ proAB lacIqlacZΔM15 Tn10 (Tcr) |
36 |
MS59 |
MC4100 supF
|
M. Syvanen collection |
JM103 |
Δ(lac-proAB) supE hsdR rpsL endA thi sbcBC/F′ traD36 proAB lacIqlacZΔM15 |
25 |
CJ236 |
dut1 ung1 thi recA pCJ105 (Cmr) |
23 |
MH760λ |
MC4100 ompR472 (λ) (thermostable λ lysogen) |
33 |
Plasmids |
|
pBH20 |
pBR322 with wild-type lacPO (Apr) |
20 |
pACS107 |
P15A ori; S107RRz cloned under lacPO control |
4 |
pACSam |
Same as pACS107 but with Sam7 null allele |
3 |
pRG1 |
pBH20 with SRRz cloned under lacPO control |
30 |
pBS112 |
S107 on high-copy-number vector with the translational initiation signals of phage T7 gene 10
|
4 |
pPF101 |
pRG1 SK92am
|
This study |
pPF102 |
pRG1 SR93am
|
This study |
pPF110 |
pRG1 S105
|
This study |
pPF113 |
pPF110 SK97am
|
This study |
pPF114 |
pPF110 SK98am
|
This study |
pPF115 |
pPF110 SE102am
|
This study |
pPF116 |
pPF110 SD103am
|
This study |
Bacteriophages |
|
λCE6 |
Sam7 cI857 int::(T7 gene 1) |
37 |
M13SRRz |
M13mp18 (carrying the λ lysis cassette SRRz) |
4 |
M13S105RRz |
M13mp18 (carrying S105RRz) |
3 |
λkan |
λ b519 b515 Tn903 cI857 nin5
|
21 |
λkan Δ(SR) |
λ b519 b515 Tn903 cI857 nin5 Δ(SR) |
31 |
λbj1 |
λkanSA52G (early lysis, non-plaque forming) |
21 |
λbj1 |
Plaque-forming pseudorevertant of λbj1 |
This study |
λS105 |
λkan SM1L (start codon for S107 inactivated; produces only S105) |
31 |