TABLE 1.
Bacterial strains, plasmids, and phages used in this study
| Strain, plasmid, or bacteriophage | Genotype | Source or reference |
|---|---|---|
| Strains | ||
| MC4100 | araD139 Δ(argF-lac)U169 thiA rpsL150 relA1 deoC1 ptsF25 rbsR flbB5301 | 33 |
| MC4100F′ | MC4100/F′ proAB lacIqlacZΔM15 Tn10 (Tcr) | 36 |
| MS59 | MC4100 supF | M. Syvanen collection |
| JM103 | Δ(lac-proAB) supE hsdR rpsL endA thi sbcBC/F′ traD36 proAB lacIqlacZΔM15 | 25 |
| CJ236 | dut1 ung1 thi recA pCJ105 (Cmr) | 23 |
| MH760λ | MC4100 ompR472 (λ) (thermostable λ lysogen) | 33 |
| Plasmids | ||
| pBH20 | pBR322 with wild-type lacPO (Apr) | 20 |
| pACS107 | P15A ori; S107RRz cloned under lacPO control | 4 |
| pACSam | Same as pACS107 but with Sam7 null allele | 3 |
| pRG1 | pBH20 with SRRz cloned under lacPO control | 30 |
| pBS112 | S107 on high-copy-number vector with the translational initiation signals of phage T7 gene 10 | 4 |
| pPF101 | pRG1 SK92am | This study |
| pPF102 | pRG1 SR93am | This study |
| pPF110 | pRG1 S105 | This study |
| pPF113 | pPF110 SK97am | This study |
| pPF114 | pPF110 SK98am | This study |
| pPF115 | pPF110 SE102am | This study |
| pPF116 | pPF110 SD103am | This study |
| Bacteriophages | ||
| λCE6 | Sam7 cI857 int::(T7 gene 1) | 37 |
| M13SRRz | M13mp18 (carrying the λ lysis cassette SRRz) | 4 |
| M13S105RRz | M13mp18 (carrying S105RRz) | 3 |
| λkan | λ b519 b515 Tn903 cI857 nin5 | 21 |
| λkan Δ(SR) | λ b519 b515 Tn903 cI857 nin5 Δ(SR) | 31 |
| λbj1 | λkanSA52G (early lysis, non-plaque forming) | 21 |
| λbj1 | Plaque-forming pseudorevertant of λbj1 | This study |
| λS105 | λkan SM1L (start codon for S107 inactivated; produces only S105) | 31 |