TABLE 2.

Lysis phenotypes of C-terminal S mutants

S allele	C-terminal sequence	Net charge	Lysis onset (min) for allelea:		Lysis rateb	Pre-mature lysisc
			S	S105
	++  ++  −− +
Wild type	..LIKRFAAKKAGVEDGRNQ	+3	35	28	Fast	+
	90                107
	++  ++  −
D103am	..LIKRFAAKKAGVE	+3		>45	Slow	+
	++  ++
E102am	..LIKRFAAKKAGV	+4		40	Slow	+
	++  +
K98am	..LIKRFAAK	+3		24	Fast	+
	++
K97am	..LIKRFAA	+2		22	Fast	+
	+
R93am	..LIK	+1	21		Fast	+
K92am	..LI	0	No	No	None	−

^aThe S allele has both wild-type translational start codons (M1, M3). The S105 allele has the M1L translational start codon (no start codon for the S107 reading frame). 

^bRate of lysis after onset. Fast, A₅₀₀ decreases by ≥50% in 5 min; slow, A₅₀₀ decreases by <50% in 15 min. 

^cLysis observed after the addition of 10 mM KCN at 5 min before the normal lysis time.