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. 2022 Jul 18;12(13):5710–5726. doi: 10.7150/thno.71832

Figure 3.

Figure 3

cTBS treatment attenuated the dead counts of neural progenitor cells and newborn neuronal cells in peri-infarct region examined at 6 and 22 days after PT stroke. (A) (a-d) Double labeling of brain slices 6 days after PT stroke indicates co-expression between SOX2 and TUNEL (yellow), and the cells were counted. (B) (a-e) Representative triple-labeled confocal images of BrdU (red), NeuN (green) and TUNEL (blue) on the sections at 6 and 22 days after PT stroke. Confocal orthogonal view of zoomed image (inset white color) in typical PT group is placed in the right panels, showing the three​-color co-localization (e). The counts of TUNEL positive newborn neurons (BrdU and NeuN double positive cells) were analyzed and shown (f). Scale bars = 50 μm. Data are expressed as mean ± SE (N = 5-8). *P < 0.05 versus Ctl group, #P < 0.05 versus PT group without cTBS.