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. 2022 Aug 8;12(13):5914–5930. doi: 10.7150/thno.75816

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Detection of Orf-cDNA in cell-free bronchoalveolar lavage fluid (cfBALF) using the magnetic-responsive biosensing platform. (A) Illustration of cfBALF collected from naïve Balb/c mice. (B) SERS spectra of the biosensing platform after detecting blank samples, cfBALF, Scr-Orf in cfBALF (1 nM), Orf-cDNA in cfBALF (1 aM), and Orf-cDNA in water (H₂O, 1 aM), displayed from bottom to top. (C) MBA-peak intensities of the biosensing platform after detecting samples, corresponding to (B). (D) Stability test of the magnetic-responsive biosensing platform on detecting Orf-cDNA under various conditions. Error bar denotes the standard deviation resulting from three independent experiments. Statistical analysis of pairwise comparison was determined by one-way ANOVA. No significance (ns): P > 0.05; * P < 0.05.