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SDS-PAGE (16% polyacrylamide) of recombinant B. subtilis CDA. Lanes: 1, size markers; 2, crude cellular extract of JF611; 3, crude cellular extract of JF611/pSO143; 4, purified recombinant CDA from step 5 of the purification in Table 1. The polypeptide bands were visualized by staining with Coomassie brilliant blue G-250.
