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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Nature. 2022 May 25;606(7914):594–602. doi: 10.1038/s41586-022-04753-7

Figure 3. CBL0137 induces Z-DNA formation in cells.

Figure 3.

a, Fluorescence intensity of Z-DNA signal after treatment of MEFs with equimolar (5μM) amounts of the indicated compounds for 18 hrs. b, WT MEFs fixed at 12 hrs post treatment with CBL0137 (5 μM) were exposed to the indicated nucleases for 45 min, before staining for Z-DNA. c, Quantification of fluorescence intensity of Z-DNA signal in b. d, Proportion of EREs and other repeats in the mouse genome (left), compared to distribution of Z22-enriched peaks following treatment with CBL0137 (1.5 μM) (right). e, Genomic distribution of L1Md T and L1Md A elements in Z22 pulldowns. f, Location of maximum Z-scores for L1Md T and L1Md A bound by Z22 in e. g, CD spectra of Z-prone B-DNA hexamer [d(Cm8mGCACGCG)/d(CGCGTGCG)] in the presence or absence of CBL0137. h, 19F NMR spectra of 8-trifluoromethyl-2′-deoxyguanosine Z-DNA d(CGCFGCG)2 in the presence of different ratios of CBL0137DNA. n = 30 per group in a, c. Two-tailed unpaired t-test with Welch’s correction (c). ***P < 0.0005 (P < 0.0001 in c). Data are representative of at least three independent experiments.