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Effect of dnaC(Ts) mutations on his-lac expression at 28 and 43°Ca
| Strain | dnaC allele | β-Galactosidase activity in Miller units (deattenuation ratio)
|
|
|---|---|---|---|
| 28°C | 43°C | ||
| MA4535 | Wild type | 93 (1.0) | 119 (1.0) |
| MA4530 | dnaC141 | 103 (1.1) | 395 (3.3) |
| MA4534 | dnaC602 | 90 (1.0) | 396 (3.3) |
Bacterial cultures were grown overnight at 28°C in nutrient broth (NB), diluted 1 to 200, and incubated at 28 or 43°C in NB. β-Galactosidase activity was determined as described in the footnote to Table 2.
