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. 2022 Jul 29;13:902932. doi: 10.3389/fmicb.2022.902932

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Copyright © 2022 Xie, Liu, Di, Liu, Gong, Chen, Li, Yu, Lv, Zhong, Song, Liao, Song, Wang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic of the construction of ASFV-ΔECM3 deletion mutant. (A) The EP153R, EP402R, MGF_360-12L, MGF_360-13L, and MGF_360-14L genes from the GZ201801 genome were replaced by the p72eGFPΔEP153R/ΔEP402R and p72mCherryΔMGF_360-12L/13L/14L recombination transfer vectors by homologous recombination. (B) eGFP and mCherry reporter fluorescence indicate BMDM cells infection with the ASFV-ΔECM3 virus. (C) Confirmation of the ASFV-ΔECM3 deletion mutant by PCR. Lanes 1 and 6 test for the EP153R gene. Lanes 2 and 7 test for the EP402R gene. Lanes 3 and 8 test for the MGF_360-12L gene. Lanes 4 and 9 test for the MGF_360-13L gene. Lanes 5 and 10 test for the MGF_360-14L gene.