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. 2022 Aug 12;17(8):e0273044. doi: 10.1371/journal.pone.0273044

Fig 5. TNBC but not HR+ BCA secrete high amounts of IL6.

Fig 5

(A) mRNA expression levels of M1 markers (CXCL10, IL-1β, and TNF-α) and M2 markers (TGFβ1, TGFβR2, CCL18, CCL22, and IL10) of MCF-7 CM-induced TAMs. (B) Heat map of the mRNA expression levels of TAMs induced by the conditioned media from MDA-MB-231, MDA-MB-468, or MCF-7, and IL-4/IL-13 polarized macrophages (M2). (C) Quantitative detection of cytokines IL4, IL10, IL6, and TNF-α from the conditioned-media of MDA-MB-231, MDA-MB-468, and MCF-7 using CBA assay. (D) The mRNA expression levels of CXCL10, IL-1β, TNF-α, and CCL22 were measured in TAMs generated from MCF-7-CM supplemented with rhIL-6 1000 pg/mL. Data are represented as mean ± SEM of three independent experiments, with significance level at *p < 0.05, **p < 0.01, ***p < 0.001, ****, and p < 0.0001. TAMs, tumor-associated macrophages; TNBC, triple-negative breast cancer; CM, conditioned media; TNF, tumor necrosis factor; IL, interleukin.