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. 2022 Aug 12;8(31):eabo6049. doi: 10.1126/sciadv.abo6049

Fig. 3. C11orf94 is stabilized by binding to the catalytic center of SPPL2c.

Fig. 3.

(A) HEK cells were transiently transfected with the indicated constructs, and C11orf94 was pulled down from the lysates using anti-FLAG and protein G agarose beads. Protein levels in lysates and immunoprecipitated (IP) samples were subjected to Western blot analysis. (B) After transfection of HEK cells with the indicated constructs, C11orf94 levels were compared between the samples by Western blot analysis. (C) Quantification of (B). N = 4, n = 8, one-way analysis of variance (ANOVA) with Tukey’s post hoc test. Throughout the figure, statistical indications above the individual bars depict significance against the empty vector (EV)–cotransfected control. (D) Processing of RAMP4-2 by SPPL2c (2c) in the presence or absence of C11orf94 (C11) was evaluated by Western blotting of lysates prepared from transiently transfected HEK cells. (E) Quantification of (D). N = 4, n = 4, one-way ANOVA with Tukey’s post hoc test. In addition to significance against the EV-cotransfected sample indicated above the individual bars, statistical comparisons of other samples are indicated with lines. *P ≤ 0.05 and **P ≤ 0.01.