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. 2022 Aug 15;132(16):e155931. doi: 10.1172/JCI155931

Figure 7. Modulation of HSATII RNA with LNA is cytotoxic, induces IFN response, and increases MHC class I expression.

Figure 7

(A) Expression levels of HSATII and other repeat RNAs in EOC cell lines transfected with HSATII-specific LNA relative to scramble control LNA over time, plotted as fold change over control on days 0 through 6 after transfection. (B) Expression heatmap depicting relative expression of innate immune-response genes and IFN-stimulated genes (ISGs) in EOC cell lines transfected with HSATII-specific LNA relative to scramble control LNA over time. (C) Effect of HSATII-specific LNA (LNA1) on tumorsphere growth in EOC cell lines, as determined by 3D CellTiter-Glo viability assays. Plots represent 4 separate experiments for each cell line, with 2-tailed unpaired t test performed at each time point. **P < 0.01, ****P < 0.0001. (D) Flow cytometric analysis of MHC-I and MHC-II cell surface protein expression on EOC cell lines transfected with LNA1 compared with control LNA. (E) Expression heatmap depicting relative expression of MHC class I genes and PD-L1 in EOC cell lines transfected with HSATII-specific LNA relative to scramble control LNA over time.