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. 2022 Aug 1;61(3):112. doi: 10.3892/ijo.2022.5402

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Overexpression of HHLA2 promotes the EMT process in gall bladder cancer cells in vitro. (A) Overexpression of HHLA2 was evaluated by western blotting and (B) quantified. (C) Expression of EMT markers Col-I, N-cadherin, α-SMA and vimentin were increased whilst that of E-cadherin was decreased in the HHLA2 overexpression group according to western blotting and (D) subsequent quantification. GAPDH was used as the internal reference protein. The data are expressed as the mean ± SD with n=3. (E) Immunofluorescence staining was utilized to evaluate the expression of EMT markers and HHLA2. Scale bars, 100 µm. Green, target protein; Blue, DAPI. ^**P<0.01 and ^***P<0.001 (HHLA2 group vs. HHLA2-NC group). HHLA2, human endogenous retrovirus-H long terminal repeat-associating protein 2; EMT, epithelial-mesenchymal transition; Col-I, collagen I; α-SMA, α-smooth muscle actin.