Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 1;61(3):112. doi: 10.3892/ijo.2022.5402

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

HHLA2 knockdown inhibits the EMT process in GBC cells in vitro. (A) HHLA2 expression was significantly decreased after transfection with the HHLA2 shRNA (n=3), (B) according to the quantification. (C) Expression of EMT markers Col-I, N-cadherin, α-SMA and vimentin were decreased whilst that of E-cadherin was increased in GBC cells transfected with the HHLA2 shRNA, (D) according to subsequent quantification. (E) Immunofluorescence staining revealed similar results to western blotting. Scale bars, 100 µm. Green, target protein; Blue, DAPI.^**P<0.01 and ^***P<0.001 (shHHLA2 group vs. sh-NC group). HHLA2, human endogenous retrovirus-H long terminal repeat-associating protein 2; EMT, epithelial-mesenchymal transition; Col-I, collagen I; α-SMA, α-smooth muscle actin; shRNA or sh, short hairpin RNA; GBC, gall bladder cancer; NC, negative control.