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. 2022 Aug 1;61(3):112. doi: 10.3892/ijo.2022.5402

Figure 6.

Figure 6

HHLA2 knockdown inhibits TGF-β1-induced proliferation, migration and invasion in the gall bladder cancer cell lines. (A) According to the wound-healing assay, (B) which was quantified, after treatment with TGF-β1 for 48 h, cell migration in the HHLA2 knockdown group was decreased compared with that of the NC group. Scale bar, 100 µm. (C) HHLA2 knockdown downregulated decreased cell invasion, (D) according to subsequent quantification. Scale bars, 100 µm. (E) The proliferative ability of cells after HHLA2 knockdown was decreased compared with that in the TGF + sh-NC group, as determined by EdU assay and (F) was quantified. Scale bars, 100 µm. Green, EdU; Blue, DAPI. **P<0.01 and ***P<0.001 (TGF + shHHLA2 group vs. TGF + sh-NC group). HHLA2, human endogenous retrovirus-H long terminal repeat-associating protein 2; shRNA or sh, short hairpin RNA; NC, negative control.