Skip to main content
. 2022 Aug 1;61(3):112. doi: 10.3892/ijo.2022.5402

Figure 8.

Figure 8

HHLA2 knockdown inhibits H19 overexpression-induced proliferation, migration and invasion in GBC cell lines. (A) From the images of wound healing assay, (B) which were quantified, HHLA2 knockdown reversed H19 overexpression-induced promotion of GBC migration. Scale bars, 100 µm. (C) After transient transfection with HHLA2 shRNA, the H19-overexpressing GBC cell line showed decreased invasive capabilities (D) following quantification. Scale bars, 100 µm. (E) Knocking down HHLA2 expression inhibited the H19 overexpression-induced cell proliferation according to EdU assay, (F) which was quantified. Scale bars, 100 µm. Green, EdU; Blue, DAPI. ***P<0.001 (H19 + shHHLA2 group vs. H19 + sh-NC group). HHLA2, human endogenous retrovirus-H long terminal repeat-associating protein 2; GBC, gall bladder cancer; shRNA or sh, short hairpin RNA; NC, negative control.