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. 2022 Aug 13;25(9):104925. doi: 10.1016/j.isci.2022.104925

Figure 3.

Figure 3

Concentration response curves for active compounds and the measurement of small molecule effect on mRNA production from the virus subgenomic promoter by FISH assay in Huh7 cells

(A) Examples of response curves are shown for the indicated active compounds using treatment concentrations ranging from 4 μM down to 0.2 nM. Each concentration was repeated in triplicate and average and standard deviations shown and normalized to vehicle (DMSO)-treated controls.

(B) Virus mRNA production was measured by in situ oligonucleotide-based detection. Examples of images are shown for the indicated compounds. Cells were stained for production of virus mRNA encoding the N protein by the smiFISH method using virus gene-specific oligonucleotides and a Cy5-labeled oligonucleotide that bound to a shared complementary region on each (red). Cell nuclei were stained with Hoechst 33342 (blue). The scale bar in the top left image is 100 μm.