REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
ACE2 Antibody	R&D Systems	Cat#AF933, RRID:AB_355722
DPP4 Antibody	R&D Systems	Cat#AF1180, RRID:AB_354651
SARS-CoV-2 N Protein Antibody	SinoBiological	Cat#40143-MM05, RRID Number: AB_2827977
Goat anti-Mouse AF488 Secondary Antibody	ThermoFisher	Cat#A28175, RRID:AB_2536161
Chicken anti-Goat AF488	Invitrogen	Cat#A21467, RRID:AB_2535870
p24gag Antibody, Clone 183-H12-5C	HIV Reagent Program (Contributor: Dr. Bruce Chesebro)	Cat#ARP3537, RRID:AB_2832923
Goat anti-Mouse HRP-conjugated Antibody	Sigma	Cat#A2554, RRID:AB_258008
Chicken anti-Goat AF488 Secondary Antibody	ThermoFisher	Cat#A-21467, RRID:AB_2535870
Bacterial and virus strains
SARS-CoV-2 WA (hCoV-19/USA-WA1/2020)	BEI	Cat#NR-52281
SARS-CoV-2 Beta (B.1.351, hCoV-19/South Africa/KRISP-K005325/2020)	BEI	Cat#NR-55282
SARS-CoV-2 Delta (B.1.617, hCoV-19/USA/MA-NEIDL-01399/2021)	Dr. John Connor	N/A
MERS-CoV (EMC/2012)	BEI	Cat#NR-44260
2019-nCoV_N_Positive Control	IDT	Cat#10006625
Biological samples
EpiIntestinal	MatTek	Cat#SMI-100
Chemicals, peptides, and recombinant proteins
Broad Institute Drug Repurposing Hub Compounds	SM Corsello, et al., 2017	https://www.broadinstitute.org/drug-repurposing-hub
NBD-PE	Invitrogen	Cat#N360
Obatoclax	MedChemExpress	Cat#HY-10969
Formamide	ThermoFisher	Cat#17899
NEBuffer 3	NEB	Cat#7003S
RNAse-Free Bovine Serum Albumin	Millipore Sigma	Cat#B6917
Ribonucleoside Vanadyl Complex	NEB	Cat#S1402S
tRNA from E. coli	Millipore Sigma	Cat#10109541001
Critical commercial assays
Luna Universal Probe One-Step RT-qPCR mixture	NEB	Cat#E3006
2019-nCoV RUO Kit	IDT	Cat#10006713
Monarch DNA Gel Extraction Kit	NEB	Cat#T1020
HiScribe T7 High Yield RNA Synthesis Kit	NEB	Cat#E2040
Monarch Cleanup Kit	NEB	Cat#T1030
Bright-Glo Luciferase Assay	Promega	Cat#E2610
LR Clonase II	ThermoFisher	Cat#11791020
Deposited data
Screening results can be found at https://clue.io/data	Broad Institute	COVID#LINCS_COVID_PUBLIC
Additional data is deposited at Zenodo https://doi.org/10.5281/zenodo.6678298	Zenodo	10.5281/zenodo.6678298
Experimental models: Cell lines
African Green Monkey Kidney: VeroE6	ATCC	Cat#CRL-1586, RRID:CVCL_0574
Human Lung Epithelial: A549	ATCC	Cat#CCL-185
Human Liver Epithelial: Huh7	JCRB	Cat#JCRB0403, RRID:CVCL_0336
Human Kidney Epithelial: HEK293FT	Invitrogen	Cat#R70007, RRID:CVCL_6911
Experimental models: Organisms/strains
K18-hACE2 Mice: B6.Cg-Tg(K18-ACE2)2Prlmn/J	Jackson Labs	Strain #034860
Oligonucleotides
2019-nCoV_N2-F 5′-GACCCCAAAATCAGCGAAAT-3′	IDT	Cat#10006713
2019-nCoV_N2-R 5′-TCTGGTTACTGCCAGTTGAATCTG-3′	IDT	Cat#10006713
probe 2019-nCoV_N2-P 5′-FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1-3′	IDT	Cat#10006713
T7 promoter-containing forward primer 5′-TAATACG ACTCACTATAGGGTAAAGGCCAACAACAACAAG-3′	Synthesized by GENEWIZ	N/A
T7 promoter-containing reverse primer 5′-GAGTCAG CACTGCTCATGGATTG-3′	Synthesized by GENEWIZ	N/A
FISH Oligonucleotides	See Table S5	N/A
Recombinant DNA
pHAGE-EF1-ACE2	Dr. Steve Elledge	N/A
pLEX307-DPP4-puro	Addgene	Cat#158451
pLP1, pLP2, pLP/VSVG	Invitrogen	Cat#K497500
SARS-CoV-2 S/gp41	Dr. Nir Hacohen	N/A
RRL.sin.cPPT.SFFV/ACE2.IRES-puro	Addgene	Cat#145839
psPAX2	Addgene	Cat#12260
pMD2.G	Addgene	Cat#12259
Software and algorithms
CellProfiler	McQuin, C. et al. 2018	https://cellprofiler.org/
Oligostan	Tsanov et al. (2016).	https://bitbucket.org/muellerflorian/fish_quant/src/master/Oligostan/
Other code is available through Zenodo https://doi.org/10.5281/zenodo.6678298	Zenodo	https://doi.org/10.5281/zenodo.6678298
Other
Cytation 1	BioTek	https://www.biotek.com/products/imaging-microscopy-cell-imaging-multi-mode-readers/cytation-1-cell-imaging-multi-mode-reader/
Ti2-E Eclipse Microscope	Nikon	https://www.microscope.healthcare.nikon.com/products/inverted-microscopes/eclipse-ti2-series
Prime BSI Camera	Photometrics	https://www.photometrics.com/products/prime-family/primebsi
LSM 700 Confocal Microscope	Zeiss	N/A
TissueLyser II	Qiagen	https://www.qiagen.com/us/products/human-id-and-forensics/automation/tissuelyser-ii/