TABLE 3.
Mutant frequencies in dnaQ+/dnaQ diploid strainsa
| Genotype | No. of Rifr mutants/106 cells |
|---|---|
| Wild type | 0.16 |
| dnaQ991/dnaQ+ | 0.15 |
| dnaQ991/dnaQ+ spq-2 | 0.41 |
| dnaQ903::tet/dnaQ+ | 0.10 |
| dnaQ903::tet/dnaQ+ spq-2 | 0.37 |
Strains were constructed as described in the text and the legend to Table 2. Cells were grown overnight at 43°C in LB broth, plated on LB and LB Rif plates, and incubated at 43°C overnight. Plating at 37°C yielded similar results.