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TABLE 3.

Mutant frequencies in dnaQ+/dnaQ diploid strainsa

Genotype No. of Rifr mutants/106 cells
Wild type 0.16
dnaQ991/dnaQ+ 0.15
dnaQ991/dnaQ+ spq-2 0.41
dnaQ903::tet/dnaQ+ 0.10
dnaQ903::tet/dnaQ+ spq-2 0.37
a

Strains were constructed as described in the text and the legend to Table 2. Cells were grown overnight at 43°C in LB broth, plated on LB and LB Rif plates, and incubated at 43°C overnight. Plating at 37°C yielded similar results.