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. 2022 Jul 8;41(33):4003–4017. doi: 10.1038/s41388-022-02391-x

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© The Author(s) 2022, corrected publication 2022

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Fig. 2 — Cell viability was measured following 24 h of PFK158 treatment at 0, 1, 2.5, 5, 10, 20 μM concentrations using MTT assay in both parental and CSC enriched tumor spheroids of (a) H1048, (b) H1882, (c) H1876 and (d) DMS53 cells. Values are expressed as the mean ± SD and experiments were conducted in triplicate (n = 3) and repeated independently three times. H1048 (e) and H1882 (g) Cells grown as spheroids were exposed to the indicated concentrations of PFK158 for 24 h and the effects on spheroids were measured (n = 5) and (f and h) Graphs depict the abundance of different size of spheroids upon PFK158 treatment in H1048 and H1882 cells, respectively. Scale bar: 200 μm, ×20 magnification. The effect of PFK158 on colony forming abilities of enriched (i) H1048 and (k) H1882 cells were assessed and (j and l) The number of colonies was counted and plotted (n = 3) in triplicates. Data are shown as mean ± SD and significance was determined comparing test samples to untreated control and expressed as *p < 0.05, **p < 0.01, ***p < 0.001.