Fig. 3.

Effect of MYCi975 on degradation of survivin a MDA-MB-453 and b MDA-MB-468 cells. Cells were incubated with DMSO or 10 μM of MYCi975 for 48 h. Harvested cell lysates were analyzed by commercial human apoptosis membranes. c Following 48 h of treatment with MYCi975, MDA-MB-453, MDA-MB-468 and BT549 cell lysates were assessed by Western blotting using an antibody against survivin. GAPDH was detected as loading control. Expression fold changes and % of survivin remaining were then calculated and graphed using GraphPad Prism 5. Data plotted are mean ± S.E.M (n = 3) and evaluated using the Student’s unpaired, two-tailed t-test