Fig. 6.

Effects of sunitinib on JIMT-1 cells. a JIMT-1 cells were treated with sunitinib for 4 h, stained with DRAQ5 and cell morphology was analyzed with the high-content analysis method. Median of the cell area is presented (± SEM). The diagram shows 3 independent experiments. Data were analyzed with one-way ANOVA and Sidak’s test. b The pictures were taken with a 20 × objective in confocal mode and representative images are shown. Scale bar is 100 µm. c JIMT-1 cells were treated with sunitinib for 4 h and adherence strength was determined with dispase assay as described in the Materials and methods section. Statistical evaluation was carried out with one-way ANOVA and Sidak’s post-hoc test. d JIMT-1 cells (300,000) were treated with sunitinib for 4 h and HER2 was detected with Western blotting. Sunitinib did not change the amount of HER2. Statistical analysis of densitometric values was carried out with one-way ANOVA followed by Sidak’s post-hoc test. e JIMT-1 cells (10,000) were pre-treated with sunitinib for 1 h, and then, cells were stained with Alexa Fluor 647 conjugated trastuzumab (red anti-HER2 antibody in JIMT-1 media) for 3 h. The green color indicates β-actin, while the nuclei were stained with DAPI. The pictures were taken with the Opera Phenix microscope (20 × air objective with 0.4 numerical aperture in non-confocal mode)