Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 12;12:13726. doi: 10.1038/s41598-022-18059-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Isolation of P. aeruginosa colony variants for assaying the genome and gene expression. (A) Diagram of the experimental methods for obtaining variant colony emergence for P. aeruginosa PAO1 with tobramycin. (B) In vitro imaging system (IVIS) images of representative phoenix colony plates show areas of bacterial sampling. Seventy-two hours post tobramycin bead placement, samples were taken from the edge of the zone of clearance (ZOC) where phoenix colonies would be expected to arise the following day after continued incubation. Ninety-six hours post tobramycin bead placement, samples were taken from phoenix colonies, VBNC-like colonies, and the outer background lawn. Additionally, samples were taken from bacterial lawns grown for 72 h that had not been exposed to antibiotics to be used as comparative controls. Red indicates high levels of metabolic activity, blue indicates low levels of metabolic activity, black indicates no metabolic activity.