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. 2022 Aug 12;12:13726. doi: 10.1038/s41598-022-18059-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Multi-dimensional scaling (MDS) plot showing clustering of isolates. An MDS plot was generated using biological coefficients of variation (BCV) from transcript counts for each isolate sample. Samples from each group cluster well together aside from the Edge samples, and the phoenix colonies and VBNC-like colonies overlap considerably. Control lawn, Edge, and Outer samples cluster on their own and separately from the Phoenix and VBNC-like samples.