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. 2022 Aug 12;12:13726. doi: 10.1038/s41598-022-18059-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Complementation of PA3626 shows rescue of the phoenix phenotype. Phoenix colony counts were obtained for wild-type P. aeruginosa PAO1, the PA3626 transposon mutant, and the PA3626 complementation strain. After complementation of the transposon mutant, phoenix colony counts returned to wild-type levels, further confirming the importance of the tRNA pseudouridine synthase D in the emergence of phoenix colonies. *p < 0.05, **p < 0.01, n = 3.