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. 2022 Aug 12;12:13722. doi: 10.1038/s41598-022-17816-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Amplification of hsa_circ_0006423. (A) hsa_circ_0006423 and divergent primers. Hsa_circ_0006423 comes from exon 4 of BCAR3, with a size of 328 nucleotides. The splice site sequence is in the forward primer. (B) Melting curves of hsa_circ_0006423 qRT-PCR products of three representative samples (the three colors represent three different samples). (C) Sanger sequencing results of hsa_circ_0006423. The arrow indicates the cyclization site. BCAR3 breast cancer anti-estrogen resistance 3, UTR untranslated region, CDS coding sequence.