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. 2022 Aug 12;256(3):57. doi: 10.1007/s00425-022-03968-2

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© The Author(s) 2022

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Fig. 6 — Fold-change in GS2 copies, expression, and protein levels on 12 plants from the MO #2 F1 population compared to three plants from an SS population. Fold-change in GS2 copies and expression was calculated using the 2^–∆∆Ct method as described in Schmittgen and Livak (2008), using Actin as internal control. Fold-change in GS2 protein levels was calculated by dividing the LFQ intensity of the sample by the average of three SS plants. Bars represent means of three technical replicates and lines represent the standard error of the mean