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. 2022 Aug 12;15:44. doi: 10.1186/s12284-022-00584-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — MoXYL1A and MoXYL1B mutants show varying degrees of sensitivity to cell wall perturbing agents. a Physical inhibitory effect of selected cell wall stress-inducing agents on the vegetative growth of the individual strains. The strain were cultured on CM media supplemented with 200 μg/mL Calcofluor White (CFW), 0.01% SDS or 200 μg/mL Congo Red (CR) for 10-days. b Quantification and statistical evaluation of the response of MoXYL1 single and double deletion mutants and the wild-type strain to different cell wall stress inducing reagents. The inhibition data was generated from five independent biological experiments with five technical replicates each. One-way ANOVA (non-parametric) statistical analysis was carried out with GraphPad Prism8 and Microsoft Excel. Error bars represent standard deviation. Inhibition rate was calculated as a percentage = (the diameter of control − the diameter of treatment)/ (the diameter of control) × 100. Single asterisk represents a significant difference (p < 0.05)